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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

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Same author

[Prokaryotic primases - structure and function].

Postepy biochemii·2019
Same author

Fidelity of DNA replication-a matter of proofreading.

Current genetics·2018
Same author

Fidelity consequences of the impaired interaction between DNA polymerase epsilon and the GINS complex.

DNA repair·2015
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A remote palm domain residue of RB69 DNA polymerase is critical for enzyme activity and influences the conformation of the active site.

PloS one·2013
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Reversal of a mutator activity by a nearby fidelity-neutral substitution in the RB69 DNA polymerase binding pocket.

Journal of molecular biology·2010
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Different behaviors in vivo of mutations in the beta hairpin loop of the DNA polymerases of the closely related phages T4 and RB69.

Journal of molecular biology·2009

Related Experiment Video

Updated: Jul 3, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

[DNA replication fidelity].

Anna Bebenek1

  • 1Pracownia Mechanizmów replikacji DNA, Zakład Biologii Molekularnej, Instytutu Biochemii i Biofizyki, PAN, Warszawa. aniab@ibb.waw.pl

Postepy Biochemii
|July 10, 2008
PubMed
Summary

DNA replication ensures genetic material is accurately copied. Key mechanisms include nucleotide selection and proofreading by replicative polymerases, crucial for cell division and inheritance.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Context:

  • DNA replication fidelity is essential for maintaining genomic integrity across cell divisions and generations.
  • Replicative polymerases are central to this process, acting as guardians of the genome.
  • These polymerases also participate in DNA repair pathways, highlighting their multifaceted roles.

Purpose:

  • To elucidate the molecular mechanisms underlying high-fidelity DNA replication.
  • To emphasize the role of structural studies in understanding polymerase conformational changes upon substrate binding.
  • To highlight the contributions of hydrogen bonding, base pair geometry, and protein-DNA interactions to replication accuracy.

Summary:

  • High fidelity DNA replication relies on precise nucleotide selection within the polymerase active site and exonucleolytic proofreading to eliminate mismatched bases.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
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Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

Related Experiment Videos

Last Updated: Jul 3, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

  • Structural insights reveal critical conformational shifts in polymerases after substrate binding, key to their accuracy.
  • Factors such as hydrogen bonding, base pair geometry, polymerase-DNA interactions, and accessory proteins are vital for ensuring replication fidelity.

    • Impact:

    • Understanding these mechanisms provides fundamental insights into genome stability and inheritance.
    • Knowledge of replication fidelity is crucial for comprehending diseases arising from replication errors, such as cancer.
    • This research informs strategies for developing therapeutic interventions targeting DNA replication processes.