[The fabrication of hepatitis B electrochemical immunosensor array]

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Context:

• Hepatitis B virus (HBV) infection remains a significant global health concern.
• Accurate and rapid diagnostics are crucial for effective Hepatitis B (HB) management.
• Existing diagnostic methods may have limitations in terms of speed, cost, or multiplexing capabilities.

Purpose:

• To develop a novel, integrated circuit-based immunosensor array for the detection of Hepatitis B surface antigen (HBsAg).
• To evaluate the performance, accuracy, and reliability of the developed eight-channel immunosensor for qualitative and quantitative HBsAg detection.
• To assess the clinical applicability of the immunosensor by comparing its performance with the established Enzyme-Linked Immunosorbent Assay (ELISA).

Summary:

• An eight-channel gold electrodes (GEs) array was fabricated using integrated circuit (IC) technology.
• The immunosensor was constructed by co-immobilizing thionine and Hepatitis B (HB) antibody onto gold electrodes via a cysteamine/glutaraldehyde linkage.
• Hepatitis B surface antigen (HBsAg) detection was achieved by monitoring the peak current decrease of thionine, enabling qualitative and quantitative analysis with high accuracy and reliability.

Impact:

• The developed immunosensor array offers a sensitive and reliable platform for Hepatitis B diagnostics.
• Synchronous detection of HBsAg across eight channels enhances diagnostic efficiency and throughput.
• Clinical validation demonstrated good agreement with ELISA, suggesting potential for widespread adoption in Hepatitis B (HB) screening and monitoring.