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Updated: Jul 3, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Usefulness of strb1 and 16S rDNA-targeted PCR for detection of Streptomyces spp. in environmental samples
Ismail Saadoun1, Raad Gharaibeh
1Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, Jordan. isaadoun@just.edu.jo
Abstract:
In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with all the samples. Additionally, specific amplification of the streptomycin-coding gene (strb1) directly from soil revealed the presence of a single DNA band of 940 bp. These results indicate that PCR-amplification of Streptomyces specific genes could be used for direct detection of streptomycin-producing Streptomyces species from soil.
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