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Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

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Differences in interleukin-1 response between engineered and native cartilage.

Eric G Lima¹, Andrea R Tan, Timon Tai

¹Department of Biomedical Engineering, Columbia University, New York, New York 10027, USA.

Tissue Engineering. Part A

|July 10, 2008

Summary

This summary is machine-generated.

Newly engineered cartilage is fragile and susceptible to joint inflammation. Preconditioning in vitro is crucial for engineered cartilage to withstand inflammatory cytokines like interleukin-1alpha (IL-1alpha) and survive in the joint environment.

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Published on: May 14, 2020

Area of Science:

Biomaterials Science
Tissue Engineering
Orthopedics

Background:

Engineered cartilage constructs are fragile initially and require time for cellular development.
Inflammatory cytokines, such as interleukin-1alpha (IL-1alpha), are present in joints and can impede engineered tissue maturation.
Native cartilage explants are used in tissue transfer but differ in resilience from engineered tissues.

Purpose of the Study:

To assess the susceptibility of nascent engineered cartilage to interleukin-1alpha (IL-1alpha) exposure.
To compare the impact of IL-1alpha on engineered cartilage versus native cartilage explants.
To determine if in vitro preconditioning enhances engineered cartilage resilience against inflammatory cytokines.

Main Methods:

Culturing bovine chondrocytes in agarose hydrogel to create engineered cartilage constructs.
Exposing engineered tissues at different developmental stages (days 0, 14, 28) and native explants to IL-1alpha (10 ng/mL).
Utilizing a chemically defined medium, with and without dexamethasone, to study the effects.

Main Results:

Early-stage engineered tissues (days 0 and 14) showed no growth upon temporary IL-1alpha exposure.
More developed engineered tissues (day 28) and native explants withstood IL-1alpha exposure without property degradation.
Dexamethasone was used in the culture medium to investigate anti-inflammatory effects.

Conclusions:

Nascent engineered cartilage is highly vulnerable to inflammatory cytokines like IL-1alpha.
In vitro preconditioning is essential for developing mechanical integrity and chemical resistance in engineered cartilage.
Sufficient preconditioning is necessary for engineered constructs to survive the joint's mechanochemical environment.