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Updated: Jul 3, 2026

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

A lipidic-sponge phase screen for membrane protein crystallization.

Annemarie B Wöhri1, Linda C Johansson, Pia Wadsten-Hindrichsen

  • 1Department of Chemical and Biological Engineering, Molecular Biotechnology, Chalmers University of Technology, SE-405 30 Gothenburg, Sweden.

Structure (London, England : 1993)
|July 10, 2008
PubMed
Summary
This summary is machine-generated.

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Structural biology advances with a new lipidic-sponge phase screen for membrane protein crystallization. This method aids in obtaining high-resolution structures of crucial eukaryotic membrane proteins, vital for drug discovery.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Drug Discovery

Background:

  • High-resolution structures of eukaryotic membrane proteins are lacking, hindering drug development.
  • Eukaryotic membrane proteins often require specific lipids for stability and successful crystallization.
  • Previous crystallization efforts often fail due to inadequate consideration of lipidic environments.

Purpose of the Study:

  • To develop a novel crystallization screen for improved membrane protein structure determination.
  • To address the challenge of crystallizing lipid-dependent membrane proteins.
  • To facilitate the structural analysis of key eukaryotic membrane proteins as drug targets.

Main Methods:

  • Development of a sparse matrix crystallization screen using 48 lipidic-sponge phase conditions.

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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jul 3, 2026

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

  • Utilizing sponge phases to create liquid lipid bilayer environments suitable for crystallization.
  • Modification of the screen by incorporating specific lipids, such as cholesterol.

    • Main Results:

    • Successfully obtained crystals of various bacterial and eukaryotic membrane proteins using the sponge phase screen.
    • Demonstrated the screen's adaptability through lipid incorporation.
    • Achieved crystal recovery from a bacterial photosynthetic core complex by adding cholesterol.

    Conclusions:

    • The lipidic-sponge phase screen is effective for crystallizing diverse membrane proteins.
    • This approach enhances the ability to determine high-resolution structures of challenging membrane proteins.
    • The developed screen offers a valuable tool for structural biology and drug discovery efforts targeting membrane proteins.