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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Chemical switch based reusable dual optoelectronic sensor for nitrite.

V Vishnuvardhan1, R Kala, T Prasada Rao

  • 1Inorganic and Polymer materials Group (CSTD), National Institute for Interdisciplinary Science & Technology, CSIR, Trivandrum 695019, India.

Analytica Chimica Acta
|July 10, 2008
PubMed
Summary
This summary is machine-generated.

A novel optical sensor detects nitrite by monitoring Rhodamine 6G absorbance changes. This reusable sensor allows for sensitive and selective nitrite determination in environmental and food samples.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemical Sensing

Background:

  • Nitrite is a crucial analyte in environmental and food safety.
  • Accurate and sensitive detection methods for nitrite are essential.
  • Existing methods may lack selectivity or require complex instrumentation.

Purpose of the Study:

  • To develop a novel optical sensor for sensitive and selective nitrite detection.
  • To enable naked-eye detection of nitrite.
  • To design a reusable chemical switch-based dual optoelectronic sensor.

Main Methods:

  • Development of an optical sensor utilizing Rhodamine 6G.
  • Spectrophotometric analysis of absorbance changes at 525 nm.
  • Optimization of sensor parameters including acid and dye concentrations, response time, and stability.
  • Investigation of spectral shifts and switching behavior with sequential additions of analytes.

Main Results:

  • The sensor exhibits a monotonous decrease in Rhodamine 6G absorbance with increasing nitrite concentration.
  • Nitrite determination is possible in the range of 0-12.18 µmol L⁻¹ with high selectivity.
  • Addition of nitrite causes a hypsochromic shift, while other anions induce a bathochromatic shift.
  • Sequential addition of nitrite and sulfamic acid results in reversible "ON" and "OFF" absorbance switching.

Conclusions:

  • A sensitive, selective, and reusable optical sensor for nitrite detection has been successfully developed.
  • The sensor allows for both instrumental and naked-eye determination of nitrite.
  • The developed sensor is suitable for monitoring trace nitrite levels in environmental and food samples.