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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

Intracellular organisms.

M Maurin¹, D Raoult

¹Unité des Rickettsies, CNRS UPRESA 6020, Université de la Méditerranée, Faculté de médecine de la Timone, 27 Bd Jean Moulin, 13385 Marseille, Cedex 5, France.

International Journal of Antimicrobial Agents

|June 1, 1997

Summary

This summary is machine-generated.

Antibiotics struggle to reach intracellular bacteria, impacting treatment efficacy. New fluoroquinolone and macrolide antibiotics show promise for intracellular infections, but more clinical data are needed.

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Area of Science:

Microbiology
Pharmacology
Cell Biology

Background:

Intracellular pathogens pose treatment challenges due to their location within host cells.
Antibiotic penetration into eukaryotic cells and intracellular pharmacokinetics are critical for efficacy.
Beta-lactam antibiotics exhibit poor penetration into host cells, limiting their effectiveness against intracellular bacteria.

Purpose of the Study:

To review the pharmacokinetic and pharmacodynamic properties of antibiotics within eukaryotic cells.
To discuss the challenges and considerations for treating intracellular bacterial infections.
To evaluate the potential of newer antibiotic classes against intracellular pathogens.

Main Methods:

Literature review of studies on antibiotic penetration and intracellular activity.
Analysis of in vitro infected cell models for assessing antibiotic efficacy.
Examination of pharmacokinetic and pharmacodynamic data for various antibiotic classes.

Main Results:

Antibiotic efficacy against intracellular pathogens depends on penetration, subcellular localization, and potential inactivation.
In vitro models provide insights but have limitations in predicting clinical outcomes.
Newer fluoroquinolone and macrolide antibiotics demonstrate promising intracellular activity in preclinical studies.

Conclusions:

Treating intracellular bacterial infections requires careful consideration of antibiotic properties beyond traditional measures.
Further clinical investigation is essential to validate the efficacy of promising new antibiotics for intracellular pathogens.
Understanding intracellular antibiotic behavior is crucial for developing effective antimicrobial strategies.