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Updated: Jul 3, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
High tolerance of methanogens in granular sludge to oxygen
M T Kato1, J A Field, G Lettinga
1Department of Environmental Technology, Wageningen Agricultural University, Bomenweg 2, Biotechnion, 6703 HD Wageningen, The Netherlands.
Abstract:
This research assessed the effect of oxygen exposure on the methanogenic activity of anaerobic granular sludges. The toxicity of oxygen to acetoclastic methanogens in five different anaerobic granular sludges was determined in serum flasks with effective gas-to-liquid volumes of 4.65 to 1. The amount of oxygen that caused 50% inhibition of the methanogenic activity after 3 days of exposure ranged from 7% to 41% oxygen in the head space. These results indicate that methanogens located in granular sludge have a high tolerance for oxygen. The most important factor contributing to the tolerance was the oxygen consumption by facultative bacteria metabolizing biodegradable substrates. Uptake of oxygen by these bacteria creates anaerobic microenvironments where the methanogenic bacteria are protected. The results also indicate that methanogens in sludge consortia still have some tolerance to oxygen, even in the absence of facultative substrate for oxygen respiration.
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