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Related Concept Videos

Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
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3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

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Published on: October 7, 2015

Glycogen storage in tissue-engineered cartilage.

Jocelyne M T Suits1, Aasma A Khan, Stephen D Waldman

  • 1Department of Chemical Engineering, Queen's University, Kingston, Ontario, Canada.

Journal of Tissue Engineering and Regenerative Medicine
|July 10, 2008
PubMed
Summary
This summary is machine-generated.

Culturing engineered cartilage in larger media volumes promotes intracellular glycogen storage. This finding is crucial for ensuring engineered cartilage

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Cartilage tissue engineering aims to create implantable tissues that can sustain metabolic activity.
  • In vivo chondrocytes utilize intracellular glycogen for metabolism, but this is not well-understood in vitro.
  • Ensuring metabolic viability is key for the success of engineered cartilage post-implantation.

Purpose of the Study:

  • To identify optimal nutrient conditions for eliciting glycogen storage in engineered cartilage.
  • To investigate the impact of glucose concentration and media volume on chondrocyte metabolism and matrix accumulation.
  • To determine if in vitro conditions can mimic in vivo glycogen storage in chondrocytes.

Main Methods:

  • Isolated bovine articular chondrocytes were cultured in scaffold-free 3D constructs for 4 weeks.
  • Constructs were exposed to varying glucose concentrations and media volumes.
  • Intracellular glycogen, glucose utilization, lactate production, and extracellular matrix accumulation were assessed.

Main Results:

  • Neither glucose concentration nor media volume significantly affected cartilaginous tissue formation.
  • Increased media volume directly correlated with higher glucose consumption and lactate production.
  • Elevated media volumes (≥1 ml/million cells) significantly increased intracellular glycogen storage.

Conclusions:

  • Culturing engineered cartilage in higher media volumes (≥1 ml/million cells) effectively elicits intracellular glycogen storage.
  • This method may enhance the metabolic capacity of engineered cartilage for improved post-implantation survival.
  • Optimizing in vitro culture conditions is vital for functional cartilage tissue engineering.