Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Batch vs Continuous Culture01:14

Batch vs Continuous Culture

Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Etiology and Antibiotic Sensitivity Pattern of Bloodstream Infection in Patients with Hematological Malignancy Having Febrile Neutropenia.

Mymensingh medical journal : MMJ·2025
Same author

Outcome of Induction Chemotherapy with the Berlin-Frankfurt-Munster-95 Regimen in Acute Lymphoblastic Leukemia Patients: A Quasi-Experimental Study.

Mymensingh medical journal : MMJ·2025
Same author

International Staging System Status and Trend of Relapse in Multiple Myeloma Cases in a Tertiary Level Health Care.

Mymensingh medical journal : MMJ·2024
Same author

Development of highly digestible animal feed from lignocellulosic biomass Part 1: Oxidative lime pretreatment (OLP) and ball milling of forage sorghum.

Translational animal science·2020
Same author

Development of highly digestible animal feed from lignocellulosic biomass Part 2: Oxidative lime pretreatment (OLP) and shock treatment of corn stover.

Translational animal science·2020
Same author

Risk of depression among Bangladeshi type 2 diabetic patients.

Diabetes & metabolic syndrome·2017

Related Experiment Video

Updated: Jul 3, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

Effect of operating conditions on solid substrate fermentation.

J Sargantanis1, M N Karim, V G Murphy

  • 1Department of Agricultural and Chemical Engineering, Colorado State University, Ft. Collins, Colorado 80521, USA.

Biotechnology and Bioengineering
|June 20, 1993
PubMed
Summary
This summary is machine-generated.

Environmental parameters significantly impact solid substrate fermentation (SSF) for protein production. Optimizing temperature and moisture profiles enhances biomass yield, crucial for efficient bioprocess control.

More Related Videos

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

Related Experiment Videos

Last Updated: Jul 3, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Solid substrate fermentation (SSF) is a key bioprocess for producing valuable compounds.
  • Controlling environmental parameters is critical for optimizing SSF performance.
  • Understanding these parameters is essential for scaling up protein production.

Purpose of the Study:

  • To investigate the effects of air flow rate, humidity, temperature, and heat transfer on SSF performance.
  • To develop a dynamic model for predicting biomass content, moisture, and temperature.
  • To determine optimal environmental profiles for enhanced protein production.

Main Methods:

  • Development of a dynamic model to simulate SSF processes.
  • Incorporation of temperature and moisture effects on growth rate and biomass yield.
  • Comparison of simulation results with experimental data.
  • Application of a technique to find optimal temperature and moisture profiles.

Main Results:

  • Evaporative cooling using airflow is highly effective for biomass temperature control.
  • Airflow rate and heat transfer coefficient influence biomass morphology but are difficult to control.
  • Simulated biomass production significantly increased with optimized temperature and moisture profiles.

Conclusions:

  • Environmental parameters, particularly temperature and moisture, are critical for SSF optimization.
  • Dynamic modeling provides valuable insights into SSF processes.
  • Implementing optimized profiles can lead to significantly improved biomass and protein production.