Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Seromuscular enterocystoplasty in rats.

F de Badiola1, J C Manivel, R Gonzalez

  • 1Department of Urologic Surgery, University of Minnesota Hospital and Clinics, Minneapolis.

The Journal of Urology
|August 1, 1991
PubMed
Summary

This study demonstrates a novel seromuscular enterocystoplasty technique in rats, using a de-mucosalized colon segment. This method successfully creates a uroepithelial lining, increasing bladder capacity without shrinkage, potentially reducing complications associated with traditional intestinal grafts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans.

Diabetologia·2011
Same author

New tissue bulking agent (polyacrylate polyalcohol) for treating vesicoureteral reflux: preliminary results in children.

The Journal of urology·2009
Same author

Primary cardiac fibrosarcoma with pulmonary metastasis in a Labrador Retriever.

Veterinary pathology·2007
Same author

14 years of experience with the artificial urinary sphincter in children and adolescents without spina bifida.

The Journal of urology·2006
Same author

Juxtaglomerular apparatus T-cell infiltration affects glomerular structure in Type 1 diabetic patients.

Diabetologia·2003
Same author

Gastroparesis following bone marrow transplantation.

Bone marrow transplantation·2001

Area of Science:

  • Urology
  • Surgical Innovation
  • Tissue Engineering

Background:

  • Traditional enterocystoplasty using intestinal segments carries risks like infection, stones, and cancer due to intestinal mucosa.
  • A new approach is needed to mitigate these complications by altering the graft's lining.

Purpose of the Study:

  • To evaluate the feasibility of creating an enterocystoplasty using a seromuscular colonic segment lined with transitional epithelium.
  • To assess the graft's stability, bladder capacity changes, and histological outcomes in a rat model.

Main Methods:

  • Augmentation enterocystoplasty was performed in 51 rats using a de-mucosalized left colon patch.
  • The serosal surface served as the internal lining for the bladder augmentation.
  • Bladder capacity and patch size were measured, and histological analysis was conducted post-sacrifice.

Related Experiment Videos

Main Results:

  • The seromuscular patch did not shrink; it increased in size from 1.82 cm² to 2.30 cm².
  • Bladder capacity significantly increased from 2.35 ml to 5.18 ml at a mean pressure of 17 cm H2O.
  • Histology confirmed a transitional epithelial lining on the serosal surface with no fibrosis or inflammation, and intact muscular layers.

Conclusions:

  • Seromuscular enterocystoplasty is a feasible technique in a rat model.
  • This method promotes uroepithelial lining and increases bladder capacity without graft shrinkage.
  • The findings suggest a promising alternative to traditional enterocystoplasty with potentially fewer complications.