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In vitro evaluation of the clastogenicity of fumagillin

Jevrosima Stevanovic1, Zoran Stanimirovic, Milena Radakovic

  • 1Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia. rocky@vet.bg.ac.yu

Environmental and Molecular Mutagenesis
|July 10, 2008
PubMed

Insights

Fumagillin, an antibiotic, shows genotoxic effects in human lymphocytes, increasing chromosome damage and reducing cell division. This indicates fumagillin is clastogenic and cytotoxic in vitro.

Area of Science:

  • Toxicology
  • Genetics
  • Cell Biology

Background:

  • Fumagillin, an antibiotic from Aspergillus fumigatus, targets microsporidia and amoeba.
  • Systemic administration of fumagillin in mammals is toxic.
  • Previous research indicated potential genotoxic effects of fumagillin.

Purpose of the Study:

  • To investigate the clastogenic effects of fumagillin on human peripheral blood lymphocytes in vitro.
  • To evaluate the cytotoxic potential of fumagillin using various cellular assays.

Main Methods:

  • Sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests were performed.
  • Human peripheral blood lymphocytes were exposed to five concentrations of fumagillin.
  • Mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated.

Main Results:

  • All tested fumagillin concentrations significantly increased SCE frequency.
  • The three highest concentrations significantly elevated structural CA and MN formation.
  • Fumagillin significantly decreased MI, PI, and NDI, indicating cytotoxicity.

Conclusions:

  • Fumagillin exhibits clastogenic effects on human lymphocytes in vitro.
  • Fumagillin demonstrates cytotoxic potential in cultured human lymphocytes.
  • The study confirms fumagillin's genotoxicity and cytotoxicity in vitro.

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