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Related Experiment Video

Updated: Jul 3, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Antigen-specific B cell detection reagents: use and quality control.

M Anthony Moody1, Barton F Haynes

  • 1Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|July 10, 2008
PubMed
Summary

Identifying antigen-specific B cells is crucial for understanding B cell development, autoimmunity, and vaccine design. This study reviews methods for detecting these cells, focusing on B cell receptors and secreted antibodies, including new reagents for HIV-1 envelope responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Immunoglobulin reactivity tests are fundamental in immunology.
  • Understanding epitope-specific B cell responses is vital for B cell development, autoimmunity, and vaccine research.
  • Synchrony between surface and secreted antibodies enables identification of antigen-specific B cells.

Purpose of the Study:

  • To provide an overview of techniques for detecting antigen-specific B cell responses.
  • To discuss recent work on reagents for probing B cell responses to HIV-1 envelope.
  • To analyze the strengths and weaknesses of flow cytometry methods for antigen-specific B cells.

Main Methods:

  • Review of reported techniques for antigen-specific B cell detection.
  • Development of reagents for B cell responses to HIV-1 envelope.

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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
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Last Updated: Jul 3, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Cell-Free Dot Blot as a Practical and Adaptable Immunoassay Platform for the Detection of Antibody Response in Human and Animal Sera
08:21

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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

  • Analysis of flow cytometry methods for antigen-specific B cells.

    • Main Results:

    • Established methods exist for detecting antigen-specific B cells via surface B cell receptor or secreted antibodies.
    • New reagents have been developed to probe B cell responses to HIV-1 envelope.
    • An analysis of various flow cytometry techniques for antigen-specific B cells is presented.

    Conclusions:

    • Accurate detection of antigen-specific B cells is essential for advancing immunology research and therapeutic development.
    • The reviewed techniques and developed reagents offer valuable tools for studying B cell responses.
    • Further analysis of flow cytometry methods will refine antigen-specific B cell detection.