Related Experiment Video
Updated: Jul 3, 2026
Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure
Published on: August 14, 2017
Impaired liver regeneration after synchronous liver and colon resection in rats
Yasutaka Hachiya1, Kazuo Chijiiwa, Hirokazu Noshiro
1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. yachiya@mri.biglobe.ne.jp
Background/Aims:
Hepatectomy combined with colectomy is the preferred treatment for patients with synchronous colorectal cancer and hepatic metastasis. However, the effects on hepatic reserve function of combining colectomy with hepatectomy are unclear. The purpose of this study was to determine liver regeneration and functions after hepatectomy with and without colectomy.
Methodology:
23 rats underwent 70% hepatectomy (Hx group) and 23 rats underwent 70% hepatectomy with ileocecal resection (HCx group), and 6 rats just after surgery (day 0) underwent simple celiotomy. On days 0, 1, 2, 3 and 7 after surgery, the remnant liver weight, DNA synthesis rate, malondialdehyde concentration, hepatic Adenosine monophosphate, ADP, ATP, serum hyaluronic acid concentration and endotoxin level in the portal blood were measured.
Results:
In HCx group, the hepatic DNA synthesis rate on day 1 (p < 0.01) and the liver weight on day 7 (p < 0.05) were significantly lower, hepatic malondialdehyde concentration on days 1 and 2 (p < 0.05) was significantly higher and serum hyaluronic acid and portal endotoxin levels on day 1 were significantly higher (p < 0.01 and p < 0.05, respectively).
Conclusion:
Addition of colectomy to hepatectomy impairs regeneration and endothelial cell function of the remnant liver; the impairment is associated with increased levels of portal endotoxin and hepatic lipoperoxide.
