Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...

DNA Replication

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...

Translesion DNA Polymerases

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...

Size and Structure of Viral Genomes

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...

Prokaryotic DNA Replication

Prokaryotic DNA Replication

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early-life exposure to the SSRI paroxetine exacerbates depression-like behavior in anxiety/depression-prone rats.

Neuroscience·2014

Same author

Moessbauer spectrometry.

Analytical chemistry·2012

Same author

Moessbauer spectroscopy.

Analytical chemistry·2010

Same author

Nanomanipulation and characterization of structural proteins.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2007

Same author

The power of silence.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2001

Same author

Mutagenesis of a cAMP response element within the latency-associated transcript promoter of HSV-1 reduces adrenergic reactivation.

Virology·1997

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Studies on a temperature-sensitive step essential to herpesvirus DNA replication.

J G Stevens¹, N L Jackson

¹UCLA School of Medicine, Department of Medical Microbiology and Immunology, Los Angeles, California 90024, USA.

|August 1, 1967

Summary

Investigating infectious bovine rhinotracheitis virus replication, a heat-sensitive step occurs one hour post-infection. This step likely involves changes to the viral deoxyribonucleic acid (DNA) or its location, influenced by increased DNA polymerase activity.

More Related Videos

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Related Experiment Videos

Last Updated: Jul 3, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Area of Science:

Virology
Molecular Biology
Biochemistry

Background:

Infectious bovine rhinotracheitis virus (IBRV) replication involves a critical heat-sensitive step.
Previous research (Stevens, 1966) identified this essential step.

Purpose of the Study:

To investigate the precise nature and timing of the heat-sensitive step in IBRV deoxyribonucleic acid (DNA) replication.
To understand the role of DNA polymerase activity in this process.

Main Methods:

Utilizing temperature "shift down" experiments to pinpoint the timing of the heat-sensitive step.
Measuring viral DNA polymerase activity at different temperatures (37°C and 42°C).

Main Results:

The heat-sensitive step was determined to occur approximately 1 hour after infection.
IBRV infection led to increased activity of a unique viral DNA polymerase at both 37°C and 42°C.

Conclusions:

The heat-sensitive step is crucial for IBRV DNA replication.
This step likely involves a modification in the intracellular location or physical state of the infecting viral DNA.
Elevated DNA polymerase activity supports the proposed mechanism.