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Shope fibroma virus assay based on enumeration of cells containing inclusion bodies
1Cancer Research, Department of Medicine and Biochemistry, Indiana University Medical Center, Indianapolis, Indiana 46207, USA.
Inclusion bodies stainable by the May-Grünwald-Giemsa and fluorescent, antibody techniques are seen after infection of HeLa, human amnion, and rabbit kidney cells with Shope fibroma virus. At 20 hours after infection the number of inclusion bodies is linearly related to the dilution of virus and the volume of inoculum. These observations provide the basis for an assay. The inclusion-body count is fast, reproducible, and more sensitive than the plaque assay. There is a quantitative relationship between the amount of virus neutralized, measured by the inclusion-body count, and the dilution of added antibody.
Inclusion bodies stainable by the May-Grünwald-Giemsa and fluorescent, antibody techniques are seen after infection of HeLa, human amnion, and rabbit kidney cells with Shope fibroma virus. At 20 hours after infection the number of inclusion bodies is linearly related to the dilution of virus and the volume of inoculum. These observations provide the basis for an assay. The inclusion-body count is fast, reproducible, and more sensitive than the plaque assay. There is a quantitative relationship between the amount of virus neutralized, measured by the inclusion-body count, and the dilution of added antibody.
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