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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
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Report: prevalence and resistance pattern of Pseudomonas aeruginosa against various antibiotics

Jamshaid Ali Khan1, Zafar Iqbal, Saeed U R Rahman

  • 1Department of Pharmacy University of Peshawar, Peshawar, Pakistan.

Pakistan Journal of Pharmaceutical Sciences
|July 11, 2008
PubMed

Insights

Pseudomonas aeruginosa infections were prevalent in Peshawar, with high resistance to common antibiotics like ampicillin and ofloxacin. This highlights an urgent need for rational antibiotic prescribing to control drug-resistant strains.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Pseudomonas aeruginosa is a significant opportunistic pathogen causing various infections.
  • Understanding its prevalence and antimicrobial susceptibility is crucial for effective treatment.

Purpose of the Study:

  • To determine the prevalence and antimicrobial susceptibility patterns of Pseudomonas aeruginosa clinical isolates.
  • To assess the resistance of Pseudomonas aeruginosa to commonly used antibiotics.

Main Methods:

  • A prospective study was conducted from January 2000 to December 2004.
  • Clinical isolates were analyzed for Pseudomonas aeruginosa presence.
  • Antimicrobial susceptibility testing was performed using the disc diffusion method and Minimum Inhibitory Concentration (MIC).

Main Results:

  • Out of 4709 positive isolates, 314 (6.67%) were Pseudomonas aeruginosa.
  • Highest infection rates were observed in orthopedic wards (24.61%) and outpatient departments (20%).
  • High resistance was noted against ampicillin (>98.4%), ampicillin/sulbactam (85.3%), co-amoxiclave (83.8%), and ofloxacin (68.4%), with least resistance to amikacin (24%).

Conclusions:

  • The study reveals a high prevalence of Pseudomonas aeruginosa and alarming antimicrobial resistance in clinical isolates.
  • Rational antibiotic prescribing and judicious use are essential to combat drug-resistant Pseudomonas aeruginosa.

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