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Related Experiment Video

Updated: Jul 3, 2026

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Published on: May 16, 2021

Acute exercise does not cause sustained elevations in AMPK signaling or expression.

Robert S Lee-Young¹, George Koufogiannis, Benedict J Canny

¹Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia. robert.s.lee-young@vanderbilt.edu

Medicine and Science in Sports and Exercise

|July 11, 2008

Summary

This summary is machine-generated.

Acute exercise increases skeletal muscle AMP-activated protein kinase (AMPK) signaling temporarily. This signaling returns to baseline within 24 hours, indicating it alone doesn't sustain muscle adaptations post-exercise.

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Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

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Activating Autophagy by Aerobic Exercise in Mice

Activating Autophagy by Aerobic Exercise in Mice

Published on: February 3, 2017

Area of Science:

Exercise Physiology
Molecular Biology
Skeletal Muscle Metabolism

Background:

AMP-activated protein kinase (AMPK) is a key regulator of cellular energy.
Understanding AMPK's response to exercise is crucial for muscle adaptation.
Previous research has not fully explored AMPK signaling beyond 3 hours post-exercise.

Purpose of the Study:

To investigate AMP-activated protein kinase (AMPK) signaling in skeletal muscle.
To measure AMPK signaling immediately, 3 hours, and 24 hours after endurance exercise.
To assess protein expression of key AMPK pathway components post-exercise.

Main Methods:

Examined AMPK signaling and protein expression in untrained individuals (4 males, 4 females).
Utilized a 60-minute moderate-intensity cycling exercise bout (63% VO2peak).
Collected muscle samples at immediate, 3-hour, and 24-hour post-exercise time points.

Main Results:

AMPK alpha2 activity and Thr172 phosphorylation increased immediately post-exercise.
ACC-beta Ser222 phosphorylation also elevated immediately after exercise.
All measured AMPK signaling markers returned to baseline by 3 and 24 hours; protein expression remained unchanged.

Conclusions:

An acute exercise bout transiently activates skeletal muscle AMPK signaling.
This acute activation is insufficient for sustained increases in AMPK signaling or protein expression.
Further stimuli may be required for long-term muscle adaptations mediated by AMPK.