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Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
08:39

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Published on: June 1, 2010

Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells

Hsin-Hui Wang1, Tzong-Yann Lee, Ching-Yuang Lin

  • 1Department of Pediatrics, Section of Nephrology, Taipei Veterans General Hospital, Taipei, Taiwan.

Kidney International
|July 11, 2008
PubMed

We previously showed that a local immune response largely composed of type 1 T cells correlated with a favorable outcome of the peritonitis associated with peritoneal dialysis. To clarify how these subsets are recruited to the peritoneal cavity during inflammation, we measured integrin-mediated interactions between the T cells and human peritoneal mesothelial cells. Direct microscopy showed that lipopolysaccharide or peritoneal dialysis effluent stimulated the adherence of T cells to mesothelial cells, a process mediated by the integrins alpha6beta1 and alpha4beta1. Further, the migration of Th1 cell across human mesothelial cell monolayers grown on transwell surfaces was reduced by anti-alpha6beta1 integrin antibody while that of Th2 cell was inhibited by an anti-alpha4 integrin antibody. Pretreatment with either lipopolysaccharide or rapid response peritoneal dialysis effluent stimulated T cell migration and this was significantly decreased by the alpha6beta1 compared to the alpha4 antibody. These results suggest that integrins may play an important role in mediating selective T cell subset adhesion and migration across human peritoneal mesothelial cell monolayers and differential integrin expression and selective T cell subset recruitment during peritonitis may affect outcome.

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