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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: Jul 3, 2026

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device
08:28

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

Phase fluorometric sterilizable optical oxygen sensor.

S B Bambot1, R Holavanahali, J R Lakowicz

  • 1Department of Chemical and Biochemical Engineering and Medical Biotechnology Center of the Maryland Biotechnology Institute, University of Maryland Baltimore County, Baltimore, Maryland 21228.

Biotechnology and Bioengineering
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

A novel optical oxygen sensor offers a low-cost, reliable alternative to Clark-type electrodes for dissolved oxygen measurement in bioreactors. This fluorescence lifetime-based sensor overcomes stability and calibration issues, enabling accurate low oxygen tension detection in cell cultures.

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Last Updated: Jul 3, 2026

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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Area of Science:

  • Biotechnology
  • Sensor Technology
  • Analytical Chemistry

Background:

  • Amperometric Clark-type electrodes are widely used for dissolved oxygen measurement but suffer from limitations.
  • These limitations include long-term stability issues, calibration drifts, and difficulties in measuring low oxygen tensions in dense microbial cultures.
  • Optical methods offer a potential alternative for dissolved oxygen sensing.

Purpose of the Study:

  • To develop and present a low-cost, optical oxygen sensor as an alternative to traditional amperometric electrodes.
  • To demonstrate the advantages of fluorescence lifetime-based optical methods for dissolved oxygen measurement.
  • To highlight the sensor's suitability for cell cultures and bioreactors.

Main Methods:

  • The sensor utilizes the differential quenching of chromophore fluorescence lifetime in response to oxygen partial pressure.
  • Measurements are based on the phase shift in fluorescence emission caused by oxygen quenching.
  • The sensor is excited by an intensity-modulated light beam.

Main Results:

  • The developed optical sensor provides a low-cost and attractive alternative to Clark-type electrodes.
  • Lifetime-based optical methods show advantages over intensity-based optical methods and amperometric electrodes.
  • The sensor is autoclavable, maintenance-free, and addresses stability and calibration drift issues.

Conclusions:

  • The fluorescence lifetime-based optical oxygen sensor is particularly suitable for dissolved oxygen measurements in bioreactors.
  • It overcomes key limitations of Clark-type electrodes, enabling reliable measurements in dense microbial cultures.
  • This sensor represents a significant advancement for dissolved oxygen monitoring in bioprocessing.