Related Experiment Video
Updated: Jul 3, 2026

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
Published on: October 17, 2013
Selective protein transport through a thin cellulose coated porous membrane
1Center for Controlled Chemical Delivery/Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84108.
Abstract:
A new composite membrane was designed and studied for permselectivity of various molecular weight proteins. The membrane is composed of a porous substrate membrane [Durapore; poly(vinylidene fluoride)] coated with a thin dense layer of regenerated cellulose. This composite membrane was fabricated by spin coating a cellulose acetate solution onto the membrane, followed by alkaline hydrolysis of the cellulose acetate coating to regenerate cellulose. The coated layer was physically characterized by scanning electron microscopy (SEM) and infrared (IR) spectroscopy. In addition, the water uptake into and permeation properties of macromolecules across the coated and uncoated membranes were studied. A typical composite membrane coating was 0.8 +/- 0.2 mum thick, resulting in a molecular weight cutoff of approximately 40,000 daltons. This composite membrane also demonstrated negligible diffusional lag time for permeants, due to the diffusional barrier. (c) 1994 John Wiley & Sons, Inc.
More Related Videos
04:40Demonstration of Membrane Transport of Histidine using Goat Intestinal Inverted Sacs: An Experiential Pedagogical Tool for Undergraduates
Published on: October 4, 2024
12:25Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
Related Concept Videos
The Significance of Membrane Transport
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Facilitated Diffusion
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
Cellular Membranes and Drug Transport
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
Overview of Protein Sorting and Transport
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
Protein Transport to the Thylakoids
Transcellular Transport of Solutes