[RAPD genetic analysis on etiological factor of mink self-biting disease]
Yumei Li1, Jiyuan Yao, Lina Ma
1College of Animal Science and Technology, Northeast Agricultural University, Ha'erbin 150030, China.
Abstract:
Self-biting is a chronic disease, which cause wound to take effect on mink growth and pelt quality. In this study, we firstly adopted RAPD (random amplification polymorphism DNA) technique based on the reproducible 26 polymorphism primers screened from 100 random primers to analyze hereditary constitution of the samples from healthy minks and self-biting minks, respectively, at molecular level to aim to discuss the causes of self-biting. The results showed that 29 straps showed polymorphism among amplified 105 straps, of which the polymorphism rate is 27.62%. Between healthy and sick mink groups, the amplified DNA fragment through different primers indicated different distribution frequency. The similarity coefficient of mink groups is 0.8471 and genetic distance (variation) index is 0.1529. Through primer S356 (whose sequence is CTGCTTAGGG), we amplified different straps between healthy and sick mink. The amplified 1000 bp DNA fragment in the sick mink groups can preliminarily serve as molecular genetic label to distinguish from healthy and sick mink groups to gradually remove the mink individual of self-biting, achieve to purify mink groups and reduce economy loss of mink breeding industry. This work provide theoretical basis for further study on molecular breeding and disease prevention of mink.
Insights
Researchers used random amplification polymorphism DNA (RAPD) to identify genetic differences in minks with self-biting disease. A specific DNA fragment can now distinguish healthy from sick minks, aiding breeding and reducing economic loss.
Area of Science:
- Animal genetics
- Molecular biology
- Veterinary science
Context:
- Self-biting is a chronic condition affecting mink growth and pelt quality.
- Understanding the genetic basis of self-biting is crucial for disease management in mink breeding.
Purpose:
- To investigate the genetic constitution of healthy and self-biting minks using molecular techniques.
- To identify potential genetic markers for distinguishing between healthy and affected mink populations.
Summary:
- Random Amplification Polymorphism DNA (RAPD) analysis was performed on healthy and self-biting minks using 26 polymorphic primers.
- A 27.62% polymorphism rate was observed among 105 amplified DNA fragments.
- A specific 1000 bp DNA fragment amplified by primer S356 was identified as a potential genetic marker distinguishing sick from healthy minks.
Impact:
- The identified genetic marker can aid in selecting against self-biting individuals, improving mink herd purity.
- This research provides a theoretical foundation for molecular breeding and disease prevention strategies in the mink industry.
- Reducing self-biting incidence can mitigate economic losses in mink farming.
