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[Establishment and evaluation of brain adenosine A2A receptors inactivation model of mice]
Shuangshuang Dai1, Hao Wang, Jianhong An
1State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Abstract:
To establish a model of inactivation adenosine A2A receptors in brain tissues of mice, we transplanted bone marrow cells (BMCs) from wild type (WT) C57BL/6 mice into A2A receptor knockout (A2A KO) C57BL/6 mice which were previously fractionated total body irradiation of 6.2 Gyx2. Six weeks later, we identified and evaluated the model. The results showed that the sexual chromagene pattern on white blood cells of recipient mice changed from female pattern to male pattern and there were 95.9% of A2AR+ cells in peripheral white blood cells of recipient mice, whereas there was no significant difference of A2AR mRNA level in brains between these recipient mice and A2AR KO mice. Furthermore, there was no significant difference of breathing frequency, brain water content and level of glutamate between the model mice and WT mice. These results indicated that we established successfully a mouse model of inactivation adenosine A2A receptors in brain tissues. This may provide a new and efficient strategy to study the effect of adenosine A2A receptors in disease and injuries of central nervous system.
Insights
Researchers successfully created a mouse model for inactivated adenosine A2A receptors in the brain. This model uses bone marrow transplantation and offers a new strategy for studying central nervous system diseases.
Area of Science:
- Neuroscience
- Immunology
- Genetics
Context:
- Adenosine A2A receptors play crucial roles in the central nervous system.
- Developing accurate animal models is essential for understanding neurological diseases.
- Previous models may not fully replicate the targeted inactivation of specific receptors in brain tissue.
Purpose:
- To establish a reliable mouse model for studying the functional inactivation of adenosine A2A receptors in brain tissues.
- To validate the efficacy of bone marrow transplantation (BMT) as a method for creating this specific receptor knockout model.
- To assess key physiological and molecular parameters in the developed model.
Summary:
- Bone marrow cells (BMCs) from wild-type (WT) mice were transplanted into adenosine A2A receptor knockout (A2A KO) mice post-irradiation.
- Successful engraftment was confirmed by changes in white blood cell sex chromosome patterns and a high percentage of A2A receptor-positive (A2AR+) cells in peripheral blood.
- Crucially, brain A2A receptor mRNA levels remained undetectable, similar to A2A KO mice, confirming targeted inactivation in the brain.
Impact:
- The study successfully established a novel mouse model for inactivated adenosine A2A receptors in brain tissue.
- This model provides an efficient strategy for investigating the role of adenosine A2A receptors in central nervous system diseases and injuries.
- The findings pave the way for further research into therapeutic interventions targeting these receptors.
