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Updated: Jul 3, 2026
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Published on: August 29, 2017
Epitope characterization of the protective monoclonal antibody VN04-2 shows broadly neutralizing activity against
Angeline P C Lim1, Steven K K Wong, Annie H Y Chan
1Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Dr, 117510, Singapore. lpeichie@dso.org.sg
Abstract:
The monoclonal antibody VN04-2 was previously shown to protect mice against lethal A/Vietnam/1203/04 H5N1 virus challenge when administered pre- and post-infection. In this study, we characterized the binding requirements of this antibody using direct binding to hemagglutinin and neutralization assays with H5N1 virus-like particles (H5N1-VLP) of eight recent H5N1 strains representing the major mutations within the 140s antigenic loop. Binding was clade independent and 3 mutations within this antigenic region are required before escape is possible, suggesting that apart from the H5N1 viruses circulating in Indonesia, VN04-2 may provide protection against H5N1 viruses from all other regions.
