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Updated: Jul 3, 2026
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Published on: September 30, 2010
[Construction of multi-epitope DNA vaccine for Toxoplasma gondii and the study on protective immunity response in
Lin Shi1, Shan Liu, Yan-Bin Cheng
1Department of Immunology and Microbiology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. shilin@mail.xjtu.edu.cn
Aim:
To construct multi-epitope DNA vaccine for Toxoplasma gondii and study its protective immunity response.
Methods:
The gene encoding six polypeptides of T. gondii, which consists of plenty of T and B epitopes, was cloned into the eucaryotic expression vector pcDNA3.1(+). BALB/c mice were vaccinated by this multi-epitope based DNA vaccine (intramuscular needle injection). The specific antibody and T cell proliferation were determined. Meanwhile, the DNA-vaccinated mice were challenged with a lethal dose of T. gondii tachyzoites for further observation.
Results:
The eukaryotic expression plasmid (pcDNA3.1/T-ME) encoding plenty of T. gondii epitopes was constructed successfully. pcDNA3.1/T-ME immunization induced T. gondii specific humoral and cellular immunity in mice. The mice immunized with pcDNA3.1/T-ME survived significantly longer than the mice in control after challenged by T. gondii RH strain infection.
Conclusion:
The multi-epitope DNA vaccine can induce the protective immunity against T. gondii infection effectively in vivo, which is a potential strategy to control T. gondii infection.
