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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jul 3, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

[mRNA expression analysis and classification of colonic biopsy samples using oligonucleotide and cDNA microarray

Orsolya Galamb¹

¹Semmelweis Egyetem, Altalános Orvostudományi Kar II., Belgyógyászati Klinika Budapest. orsg1@yahoo.com

|July 12, 2008

Summary

Researchers identified novel biomarkers for colorectal cancer progression. Gene expression analysis revealed ten new markers that increase with adenoma-dysplasia-carcinoma sequence development, aiding in early detection and classification.

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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Area of Science:

Molecular biology
Oncology
Genomics

Context:

Colorectal cancer diagnosis and therapy still present challenges.
Gene expression analysis is crucial for understanding disease progression and classification.

Purpose:

To identify novel biomarkers for colorectal carcinoma development.
To classify colorectal diseases using gene expression profiles.
To validate gene expression changes using real-time PCR.

Summary:

Oligonucleotide microarray and Taqman microfluidic card systems were used for gene expression analysis.
Osteopontin and osteonectin mRNA/protein overexpression correlate with colorectal adenoma-dysplasia-carcinoma sequence progression.
Ten novel markers with increasing mRNA expression during tumor progression were identified and validated.
Gene expression profiles associated with adenoma, colorectal cancer, and inflammatory bowel diseases were identified.

Impact:

Provides novel molecular markers for improved colorectal cancer diagnosis and follow-up.
Enhances the classification of colorectal diseases through gene expression profiling.
Establishes reproducible and cost-efficient methods for biomarker validation.