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Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
07:38

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Broth recycle in a yeast fermentation.

T Y Hsiao1, C E Glatz, B A Glatz

  • 1Department of Chemical Engineering, 231 Sweeney Hall, lowa State University, Ames, lowa 50011.

Biotechnology and Bioengineering
|November 20, 1994
PubMed
Summary
This summary is machine-generated.

Recycling fermentation broth significantly reduces wastewater volume by up to 64%. This method sustains cell mass and lipid production in defined media but requires ion exchange for whey-based media to prevent inhibition.

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Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Biochemical Engineering

Background:

  • Fermentation processes generate substantial wastewater effluent.
  • Minimizing effluent discharge is crucial for improving process economics and sustainability.
  • Recycling fermentation broth offers a potential solution to reduce water usage and waste.

Purpose of the Study:

  • To investigate the feasibility of recycling spent fermentation broth for Apiotrichum curvatum.
  • To assess the impact of broth recycling on cell mass and lipid production.
  • To identify and address limitations associated with recycling fermentation broth.

Main Methods:

  • Apiotrichum curvatum was cultured in defined medium and whey permeate.
  • Spent broth was recycled to replace water and salts in subsequent fermentation batches (up to 75%).
  • Ion accumulation was analyzed, and ion exchange was employed to mitigate inhibition.

Main Results:

  • Recycling up to 75% of spent broth in defined medium supported consistent cell mass and lipid production for seven cycles, achieving a 64% wastewater reduction.
  • In whey permeate medium, lipid production decreased after multiple recycle operations at 50-100% recycle rates.
  • Ion accumulation was identified as the inhibitory factor, which was successfully reversed by ion exchange.

Conclusions:

  • Spent fermentation broth recycling is a viable strategy for reducing wastewater in defined media fermentations.
  • Whey permeate fermentation requires management of ion accumulation for successful broth recycling.
  • Ion exchange is an effective method to overcome inhibition caused by ion buildup during broth recycling.