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Biodegradation process development using a bacterial cytochrome in vivo.

J B Horowitz1, V L Vilker

  • 1Department of Chemical Engineering, University of California, Los Angles, California 90024.

Biotechnology and Bioengineering
|June 20, 1994
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Summary
This summary is machine-generated.

Pseudomonas putida PpG786 shows promise for hazardous hydrocarbon detoxification. Its organohalide degradation activity, influenced by heme content and oxygen, is sufficient for industrial wastewater treatment in sequencing batch reactors.

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Area of Science:

  • Environmental Microbiology
  • Biotechnology
  • Bioremediation

Background:

  • Pseudomonas putida PpG786 possesses the inducible cytochrome P-450(cam) enzyme system.
  • This bacterium is being explored for its potential in detoxifying hazardous hydrocarbons.

Purpose of the Study:

  • To assess the organohalide degradation activity of P. Putida PpG786 using 1,2-dibromochloropropane (DBCP) as a model substrate.
  • To investigate factors influencing the bacterium's detoxification capabilities and biomass stability.

Main Methods:

  • Utilized P. Putida PpG786 for degradation experiments with DBCP.
  • Varied intracellular heme content, cell culturing/processing conditions, and oxygen tension.
  • Studied biomass lifetime in chemostat washout operations, including substrate removal and reintroduction.

Main Results:

  • Degradation activity was significantly dependent on intracellular heme content, cell handling, and oxygen levels.
  • The active biomass demonstrated a slow decay rate under tested conditions.
  • Initial biomass activity was found to be sufficiently high for practical applications.

Conclusions:

  • P. Putida PpG786 exhibits promising capabilities for hazardous compound removal from industrial wastewater.
  • The bacterium's stability and activity profile are suitable for implementation in sequencing batch reactors (SBRs).