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High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

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[Study on chemical constituents isolated from Juncus effuses].

Cheng-Ying Shan¹, Yong-Hao Ye, Hong-Fang Jiang

¹Nanjing Institute for the Comprehensive Utilization of Wild Plant, Nanjing 210042, China. shancy81@hotmail.com

Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials

|July 16, 2008

Summary

This study identified eight compounds from Juncus effuses Linn., including flavonoids, aromatic compounds, a fatty acid, and an alkane. Several compounds were isolated from this plant for the first time.

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Area of Science:

Phytochemistry
Natural Product Chemistry

Background:

Juncus effuses Linn. is a plant with potential medicinal properties.
Understanding its chemical constituents is crucial for further research.

Purpose of the Study:

To isolate and identify chemical compounds from Juncus effuses Linn.
To determine novel compounds previously unrecorded in this plant species.

Main Methods:

Compounds were isolated using chromatographic techniques, including Silica gel column chromatography and Sephadex LH-20.
Compound identification was achieved through comprehensive spectroscopic analyses (UV, MS, 1H-NMR, 13C-NMR, NOE).

Main Results:

Eight compounds were successfully isolated and identified.
These included four flavonoids (luteolin, eriodictyol, 2', 5', 5, 7-tetrahydroxyflavone, glucoluteolin), two aromatic compounds (methyl p-hydroxybenzoate, vanillic acid), one fatty acid (hexadecanoic acid), and one alkane (n-Tetradecane).

Conclusions:

Several compounds, including eriodictyol, 2', 5', 5, 7-tetrahydroxyflavone, methyl p-hydroxybenzoate, vanillic acid, hexadecanoic acid, and n-Tetradecane, were isolated from Juncus effuses Linn. for the first time.
This research expands the known phytochemical profile of Juncus effuses Linn.