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Related Experiment Video

Updated: Jul 3, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

[Immunoresonance scattering spectral assay of complement factor 4(C4)].

Ai-Hui Liang1, Su-Mei Wang

  • 1Department of Material and Chemical Engineering, Guilin University of Technology, Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Ministry of Education, Guilin 541004, China. ahliang@glite.edu.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|July 16, 2008
PubMed
Summary

A new resonance scattering method was developed to detect C4 in human blood serum. This simple, rapid, and sensitive technique accurately measures C4 levels, showing high selectivity.

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Area of Science:

  • Biochemistry and Immunology
  • Analytical Chemistry
  • Nanotechnology

Context:

  • Complement factor 4 (C4) plays a crucial role in the immune system.
  • Accurate C4 quantification is essential for diagnosing various diseases.
  • Existing methods for C4 determination may have limitations in sensitivity or speed.

Purpose:

  • To develop a novel, highly sensitive, and selective method for determining C4 in human blood serum.
  • To utilize the resonance scattering (RS) effect of immune complex particles for C4 detection.
  • To optimize reaction conditions and validate the method's performance.

Summary:

  • A new resonance scattering (RS) method was developed for C4 determination in human blood serum by analyzing immune complex particles formed between goat anti-human C4 and C4.

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Last Updated: Jul 3, 2026

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06:29

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  • The method relies on the RS peaks at 350 and 390 nm, showing a linear relationship with C4 concentration (0.18–2.60 µg/mL) and a low detection limit (0.084 µg/mL).
  • Optimization of pH, reagent concentrations, temperature, and time, along with assessment of interfering substances, ensured method robustness. The method demonstrated high sensitivity, selectivity, and accuracy, comparable to immunoturbidity.

    • Impact:

    • Provides a simple, rapid, and highly sensitive analytical method for C4 detection in clinical diagnostics.
    • Offers a valuable tool for the diagnosis and monitoring of C4-related immunological disorders.
    • The use of resonance scattering of immune complexes opens new avenues for immunoassay development.