Related Experiment Video
Updated: Jul 3, 2026
Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
Published on: March 17, 2023
[Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts]
Hui-Ping Ma1, Zheng-Ping Jia, Ke-Ming Cheng
1Department of Pharmacy, Lanzhou General Hospital, PLA, Lanzhou 730050, China.
Objective:
To investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).
Method:
TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.
Result:
TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.
Conclusion:
The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.
