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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Updated: Jul 3, 2026

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Two-hybrid technologies in proteomics research.

Bernhard Suter1, Saranya Kittanakom, Igor Stagljar

  • 1Department of Biochemistry and Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research (DCCBR), University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.

Current Opinion in Biotechnology
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

Protein-protein interactions (PPIs) are crucial for life. The yeast two-hybrid (YTH) system and its variants are vital tools for studying these interactions, aiding disease understanding and drug discovery.

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Last Updated: Jul 3, 2026

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Protein-protein interactions (PPIs) are fundamental to nearly all biological processes.
  • Understanding PPI networks is critical for advancing disease research and drug discovery.
  • Robust methodologies for assessing physical protein associations are essential in proteomics.

Purpose of the Study:

  • To provide an overview of current applications of the yeast two-hybrid (YTH) system and its variants.
  • To highlight the significance of YTH-based methods in yeast and mammalian systems.
  • To discuss the future role of YTH in interactome analysis and therapeutic development.

Main Methods:

  • Overview of the yeast two-hybrid (YTH) system.
  • Discussion of variant technologies for PPI assessment.
  • Application of YTH in both yeast and mammalian cell systems.

Main Results:

  • The yeast two-hybrid system is a widely used and successful method for assessing PPIs.
  • YTH and its variants are applicable across different biological systems.
  • These methods are instrumental in mapping protein interaction networks.

Conclusions:

  • YTH-based methods will continue to be dominant in protein interactome assessment.
  • These techniques are crucial for developing novel therapeutic compounds targeting protein interaction interfaces.
  • The study underscores the importance of PPI research for future biomedical advancements.