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Updated: Feb 23, 2026

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Bimolecular fluorescence complementation in structural biology.

Young-Hwa Song¹, Matthias Wilmanns

¹EMBL-Hamburg, Notkestrasse 85, D-22603 Hamburg, Germany. song@embl-hamburg.de

Methods (San Diego, Calif.)

|July 16, 2008

Summary

This summary is machine-generated.

Bimolecular fluorescence complementation (BiFC) is a powerful technique for studying protein interactions in cells. It validates 3D protein structures and assesses homology models, advancing structural biology research.

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Bimolecular Fluorescence Complementation

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Area of Science:

Structural biology
Biophysics
Molecular biology

Background:

Protein-ligand interactions are crucial for cellular function.
Understanding protein complex structures is vital in biological research.
Existing methods may have limitations in probing interactions under physiological conditions.

Purpose of the Study:

To outline and assess recent structure-based applications of bimolecular fluorescence complementation (BiFC).
To demonstrate the utility of BiFC in validating 3D protein structures and homology models.
To highlight BiFC's potential as a leading analysis tool in structural biology.

Main Methods:

Utilizes bimolecular fluorescence complementation (BiFC) to probe protein-ligand interactions.
Applies BiFC to study homo- and hetero-oligomeric assemblies, including non-protein ligands.
Examines conformation-dependent protein complex monitoring using BiFC.

Main Results:

BiFC effectively probes protein-ligand interactions under physiological conditions.
The method successfully validates experimental 3D structures and assesses homology models.
BiFC demonstrates potential in monitoring protein complex conformation.

Conclusions:

Bimolecular fluorescence complementation is a state-of-the-art tool for structural biology.
BiFC provides independent evaluation and characterization of higher-order protein complexes.
The technique offers significant power for advancing the understanding of protein interactions.