Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity separation and enrichment methods in proteomic analysis.

Xiangming Fang1, Wei-Wei Zhang

  • 1Acrotics Corporation, San Diego, CA 92130 USA. xfang@acrotics.com

Journal of Proteomics
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gestational Arsenic Exposure Induces Metabolic Dysfunction-Associated Fatty Liver Disease-like Phenotypes in Middle-Aged Offspring.

Environment & health (Washington, D.C.)·2026
Same author

Divergent Trajectories of the Burden of MASLD Attributable to High Fasting Plasma Glucose in China and Globally: A GBD Study 1990-2021 and Projections to 2046.

Gastroenterology research and practice·2026
Same author

Comprehensive Characterization of 98 Chinese Cases of Genetic Creutzfeldt-Jakob Disease With T188K Mutation.

Annals of clinical and translational neurology·2026
Same author

Disruptions in RNA Splicing: A Key Regulator of Cognitive Impairment in Perioperative Neurocognitive Disorders.

Neuroscience bulletin·2026
Same author

[The Prevalence and Epidemiological Characteristics of Monoclonal Gammopathy of Undetermined Significance in the Healthy Individuals of Daqing Region].

Zhongguo shi yan xue ye xue za zhi·2026
Same author

Palliative care in patients with brain metastases from breast cancer: a qualitative descriptive study of attitudes, practices, and barriers.

BMC palliative care·2025
Same journal

Transpulmonary proteomic gradient analysis in women with pulmonary arterial hypertension associated with systemic sclerosis.

Journal of proteomics·2026
Same journal

Multiomic insights into fungal polylactic acid degradation: Metabolic adaptation and hydrolytic mechanisms of Sporobolomyces pararoseus.

Journal of proteomics·2026
Same journal

Temporal proteomic analysis reveals a three-phase adaptation strategy in Phytophthora cinnamomi during salinity stress.

Journal of proteomics·2026
Same journal

Proteomic and phosphoproteomic profiles of time-dependent dynamic changes in LPS-induced macrophage polarization.

Journal of proteomics·2026
Same journal

From prediction to mechanism: Explainable AI uncovers plasma and CSF proteomic signatures of Alzheimer's disease.

Journal of proteomics·2026
Same journal

Twenty years of the Mexican Proteomics Society.

Journal of proteomics·2026
See all related articles

Efficiently separating highly-abundant proteins (HAP) is crucial for detecting low-abundant proteins (LAP) in proteomic studies. Affinity-based methods offer promising solutions for protein enrichment and biomarker discovery.

Area of Science:

  • Proteomics and Systems Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Protein separation is a bottleneck in proteomic studies, hindering the detection of low-abundant proteins (LAP).
  • Highly-abundant proteins (HAP) often mask the signals of LAPs in complex biological samples like plasma and serum.
  • Effective enrichment strategies are essential for biomarker discovery and validation.

Purpose of the Study:

  • To review and contextualize affinity-based technologies for protein subtraction and enrichment in proteomics.
  • To explore the concept of 'Separomics' within the broader fields of proteomics and systems biology.
  • To provide a prospective view of affinity-mediated proteomics and discuss available products.

Main Methods:

  • Review of immunoaffinity subtraction using polyclonal IgY or IgG antibodies.

Related Experiment Videos

  • Discussion of alternative affinity methods employing recombinant proteins, lectins, peptides, and chemical ligands.
  • Analysis of methods for HAP removal and LAP capture in biological fluids.

    • Main Results:

    • Immunoaffinity subtraction methods demonstrate significant advantages for plasma and serum proteomic analysis.
    • Various affinity ligands provide diverse strategies for LAP capture and enrichment.
    • The development of 'Separomics' provides a framework for understanding protein separation technologies.

    Conclusions:

    • Affinity-based approaches are critical for overcoming limitations in protein separation for proteomics.
    • These methods are vital for advancing biomarker discovery and enabling comprehensive proteomic and systems biology research.
    • Further development and application of affinity-mediated proteomics hold significant promise for biological and medical research.