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Interplay between MgtC and PagC in Salmonella enterica serovar Typhimurium

Eric Alix1, Tsuyoshi Miki, Christine Felix

  • 1Inserm, ESPRI 26, Avenue J.F. Kennedy, 30908 Nîmes Cedex 02, France.

Microbial Pathogenesis
|July 16, 2008
PubMed

Insights

The MgtC protein in Salmonella Typhimurium impacts PagC levels and intramacrophage survival. A functional Nramp1 protein limits MgtC mutant multiplication within host cells.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Host-Pathogen Interactions

Background:

  • The PhoP-PhoQ system regulates Salmonella enterica serovar Typhimurium virulence under magnesium deprivation.
  • MgtC and PagC proteins are regulated by PhoP-PhoQ and implicated in intramacrophage survival, though their functions are unclear.

Purpose of the Study:

  • To investigate the functional relationship between MgtC and PagC in Salmonella Typhimurium.
  • To determine the role of MgtC and PagC in intramacrophage survival.
  • To assess the influence of Nramp1 on the virulence of mgtC and pagC mutants.

Main Methods:

  • Generating and analyzing DeltamgtC and pagC null mutant strains of Salmonella Typhimurium.
  • Assessing bacterial growth and survival in magnesium-depleted media and within macrophages.
  • Evaluating mutant attenuation in Nramp1-positive and Nramp1-negative mouse models.

Main Results:

  • DeltamgtC mutant showed decreased PagC levels in magnesium-depleted medium.
  • PagC did not contribute to intramacrophage survival, and pagC null mutants were poorly attenuated in mice.
  • MgtC null mutants were significantly more attenuated in Nramp1-positive mice compared to Nramp1-negative mice.

Conclusions:

  • MgtC influences PagC expression but not intramacrophage survival.
  • Nramp1 (Slc11a1) plays a crucial role in limiting Salmonella mgtC mutant proliferation within the host.
  • The function of PagC in Salmonella virulence remains to be elucidated.

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