Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rationale and current status of fecal microbiota transplantations for Parkinson's disease.

Journal of Parkinson's disease·2026

Same author

DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

Journal of bacteriology·2026

Same author

RNA-based CLEM (RCLEM) bridging RNA localisation and ultrastructural mapping in 3D.

Journal of microscopy·2026

Same author

Ferroptosis-armed dendritic cell vaccines for glioma immunotherapy.

Nature communications·2026

Same author

Faecal microbiota transplant for Parkinson's disease.

Brain : a journal of neurology·2026

Same author

Systemic Inflammation Modulates Clearance and Drives Extra-Hepatic Distribution of Extracellular Vesicles.

Journal of extracellular vesicles·2026

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

Maintaining the silence: reflections on long-term RNAi.

Koen Raemdonck¹, Roosmarijn E Vandenbroucke, Joseph Demeester

¹Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.

Drug Discovery Today

|July 16, 2008

Summary

RNA interference (RNAi) shows promise for treating genetic disorders. Future research must focus on the safe, long-term application of RNAi therapeutics for sustained patient benefit.

More Related Videos

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Related Experiment Videos

Last Updated: Jul 3, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Area of Science:

Biotechnology
Molecular Biology
Genetics

Background:

RNA interference (RNAi) has been demonstrated in mammalian cells.
Significant research and financial investment have been directed towards developing RNAi as a therapeutic platform.
RNAi is considered a highly promising strategy for treating human genetic disorders.

Purpose of the Study:

To highlight the potential of RNA interference (RNAi) as a therapeutic modality.
To emphasize the need for a shift in research focus towards the safe and long-term application of RNAi drugs.
To underscore the importance of addressing chronic treatment requirements for RNAi therapies.

Main Methods:

This study is a review and perspective based on existing research in RNA interference.
It synthesizes current understanding of RNAi technology and its therapeutic implications.
Focuses on the strategic considerations for clinical translation.

Main Results:

RNA interference is a leading therapeutic strategy for genetic disorders.
The successful implementation of RNAi therapeutics necessitates a focus on long-term safety and efficacy.
Chronic treatment paradigms are essential for many RNAi-targeted genetic conditions.

Conclusions:

Continued development of RNAi therapeutics is crucial for treating genetic diseases.
Prioritizing safe and long-term applications will enable RNAi to fulfill its therapeutic potential.
Addressing the challenges of chronic RNAi drug delivery and management is paramount.