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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Related Experiment Video

Updated: Jul 3, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Synchronized, continuous-flow zone electrophoresis.

Dawid R Zalewski1, Dietrich Kohlheyer, Stefan Schlautmann

  • 1MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500AE Enschede, The Netherlands. D.R.Zalewski@utwente.nl

Analytical Chemistry
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel continuous electrophoretic separation method for microscale devices, eliminating mechanical pumps for electrokinetic fluid transport and separation. This technique allows for sample fractionation and purification, demonstrating feasibility for on-chip integration.

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
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Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Last Updated: Jul 3, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Traditional electrophoresis often requires external pumping mechanisms.
  • Integrating separation processes into microscale devices presents challenges for continuous operation.

Purpose of the Study:

  • To develop a continuous electrophoretic separation method for microscale devices.
  • To demonstrate electrokinetic fluid transport and separation without mechanical pumps.
  • To assess the potential for on-chip integration and sample purification.

Main Methods:

  • Fabrication of microchip devices with a 10-microm-deep, 1.5 mm x 4 mm separation chamber in glass.
  • Utilizing a standard microchip electrophoresis setup.
  • Employing electrokinetic forces for both fluid transport and separation.

Main Results:

  • Continuous separation of rhodamine B, rhodamine 6G, and fluorescein was successfully achieved.
  • Demonstrated tenfold purification of a mixture containing rhodamine B and fluorescein.
  • Achieved recovery of both separated fractions, confirming method feasibility.

Conclusions:

  • The proposed method offers a pump-free approach for continuous electrophoretic separation in microdevices.
  • This technique is highly suitable for integration into multi-step analytical systems on a chip.
  • The results validate the feasibility of electrokinetic-driven continuous separation and purification at the microscale.