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07:56
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Published on: August 6, 2016
A simple and effective method to overcome the inhibition of Fe to PCR
Fei Teng1, Yuntao Guan, Wanpeng Zhu
1Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Journal of Microbiological Methods
|July 16, 2008
Abstract:
Fe inhibition to PCR can be overcome by adding greatly excessive EDTA first, and then the same amount Mg(2+) as EDTA to PCR mix. By this method impure DNA was amplified successfully. DNA samples containing metals whose complexation constant with EDTA was much higher than Mg(2+) can also be amplified.
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