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Updated: Jul 3, 2026

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining
Published on: September 10, 2021
Ultrastructural studies on accessory nuclei in developing oocytes of the crustacean, Siphonophanes grubei
J Kubrakiewicz1, R T Adamski, S M Bilinski
1Department of General Zoology, Zoological Institute, University of Wroclaw, 50-335 Wroclaw, ul. Sienkiewicza 21, Poland.
Abstract:
Numerous accessory nuclei (AN) occur within peripheral cytoplasm of Siphonophanes grubei oocytes. They exhibit typical nuclear structure, with double membrane envelope pierced by pore complexes. Although no explicit evidence was shown, indirect arguments exist indicating that AN originate from the oocyte nucleus. Presence of clumps of DNP material within AN suggests that AN might play some role in amplified DNA metabolism. Participation of AN in cellular membrane elaboration is also postulated.
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