Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of Coffee Silver Skin and Brewers' Spent Grain in the Control of Root-knot Nematodes.

Helminthologia·2019

Same author

Nitric oxide and hydrogen peroxide: two players in the defence response of tomato plants to root-knot nematodes.

Communications in agricultural and applied biological sciences·2012

Same author

Molecular and physiological characteristics of a grape yeast strain containing atypical genetic material.

International journal of food microbiology·2010

Same author

Characterization of Anionic Peroxidases in Tomato Isolines Infected by Meloidogyne incognita.

Journal of nematology·2009

Same author

The influence of lycopene on the proliferation of human breast cell line (MCF-7).

Toxicology in vitro : an international journal published in association with BIBRA·2006

Same author

Synthesis, analytical characterization and bioactivity of Ag and Cu nanoparticles embedded in poly-vinyl-methyl-ketone films.

Analytical and bioanalytical chemistry·2005

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Published on: August 16, 2019

Ultrastructural studies on the nematode Xiphinema diversicaudatum: Egg shell formation.

T Bleve-Zacheo¹, M T Melillo, G Zacheo

¹Istituto di Nematologia Agraria, via Amendola 165/ A, 70126 Bari, Italy.

|June 1, 1993

Summary

The ultrastructure of the Xiphinema diversicaudatum nematode egg shell formation is detailed, revealing a multi-layered structure. Key layers, including vitelline, chitinous, and lipid, are formed through complex secretion and extrusion processes.

More Related Videos

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

Published on: October 8, 2020

Related Experiment Videos

Last Updated: Jul 3, 2026

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Published on: August 16, 2019

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

Published on: October 8, 2020

Area of Science:

Nematology
Cell Biology
Reproductive Biology

Background:

The longidorid nematode Xiphinema diversicaudatum is an economically important plant pathogen.
Understanding nematode egg shell formation is crucial for developing effective control strategies.

Purpose of the Study:

To describe the ultrastructure of egg shell formation in Xiphinema diversicaudatum.
To elucidate the origin and deposition of the different egg shell layers.

Main Methods:

Transmission electron microscopy was used to examine the ultrastructure of developing nematode eggs.
Detailed observation of cellular processes involved in egg shell secretion and assembly.

Main Results:

The vitelline membrane forms upon fertilization, followed by secretion of the chitinous layer in the perivitelline space.
The lipid layer is extruded from the egg cytoplasm via finger-like projections, with both layers originating from cytoplasmic granules.
Additional uterine layers are deposited in the oviduct and uterus, with potential egg pores identified between uterine layers.

Conclusions:

The Xiphinema diversicaudatum egg shell is a complex, multi-layered structure formed through sequential secretion and extrusion processes.
Oviduct and uterine secretions play significant roles in enriching and completing the egg shell.
The study provides a detailed ultrastructural basis for nematode egg shell development.