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Related Experiment Video

Updated: Jul 3, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
07:32

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Published on: June 9, 2015

Sugarcane somatic embryogenesis: a scanning electron microscopy study.

S Rodríguez1, C Mondéjar, M E Ramos

  • 1Electron Microscopy Laboratory, National Center for Scientific Research, Havana, Cuba. mossquiminfomed@gn.apc.org

Tissue & Cell
|April 1, 1996
PubMed
Summary
This summary is machine-generated.

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Sugarcane plant regeneration primarily occurs through somatic embryogenesis from callus cultures. Scanning electron microscopy distinguished between embryogenic and non-embryogenic callus, detailing key developmental stages.

Area of Science:

  • Plant Biotechnology
  • Agricultural Science
  • Cell Biology

Background:

  • Efficient plant regeneration is crucial for sugarcane (Saccharum officinarum) crop improvement.
  • Distinguishing between embryogenic and non-embryogenic callus is vital for optimizing regeneration protocols.

Purpose of the Study:

  • To comparatively analyze embryogenic and non-embryogenic sugarcane callus using scanning electron microscopy (SEM).
  • To describe the sequential process of plant regeneration from sugarcane callus via somatic embryogenesis.

Main Methods:

  • Sugarcane spindles (CUBA 87-51) were cultured on Murashige and Skoog (MS) medium.
  • Scanning electron microscopy (SEM) was employed to examine callus morphology.
  • Regeneration medium (MS without 2,4-D) was used to induce plantlet development from embryogenic callus.

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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

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Last Updated: Jul 3, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
07:32

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Published on: June 9, 2015

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

Main Results:

  • SEM revealed distinct morphological differences: embryogenic callus showed organized cells forming embryos, while non-embryogenic callus had disorganized, elongated cells.
  • Sugarcane embryoid development stages were identified: globular, globular with lateral notch, and scutellum.
  • The process involved the formation of shoot and root primordia, culminating in true leaves and roots.

Conclusions:

  • Callus plant regeneration from sugarcane young leaf segments predominantly occurs via somatic embryogenesis.
  • SEM is an effective tool for differentiating sugarcane callus types and understanding regeneration pathways.