Fluorescent nanocrystal-encoded microbeads for multiplexed cancer imaging and diagnosis

Bead-based assays on very large numbers of molecules in proteomics, genomics, drug screening and clinical diagnostics require encoding of each of the microbeads according to the particular ligand bound to its surface. The benefits of using optically encoded microbeads (instead of the solid-state two-dimensional arrays) are derived from the freedom of bead to move in three dimensions. Polymeric beads optically encoded with organic dyes allow for a limited number of unique codes whereas the use of semiconductor nanocrystals as fluorescent tags improves the beads multiplexed imaging capabilities, photostability and sensitivity of the antigen detection. Additionally, an employment of the recently demonstrated Förster resonance energy transfer (FRET) from the microbeads nanocrystal codes to the nearby antibody dye label allows for the very specific detection of the interaction between the microbead and the antibody. This interaction turns the fluorescence signal from dye label off and on thus effectively discriminating between the occurrence and the non-occurrence of antibody binding. The absence of fluorescent background from non-interacting with the beads dye-labelled antibodies additionally increases the sensitivity of detection and further facilitates the multiplexing capabilities of nanocrystals-based detection and diagnostics. This paper reviews the state-of-the-art results of development of microbeads optically encoded with the fluorescent nanocrystals "quantum dots" and their applications to proteomics for cancer antigens and autoantibodies imaging and diagnosis.