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The polymerization process that involves carbanion as an intermediate is called anionic polymerization. It is also a type of addition or chain-growth polymerization. Anionic polymerization gets initiated by a strong nucleophile such as an organolithium or a Grignard reagent. The most commonly used initiator for anionic polymerization is butyl lithium. Monomers involved in anionic polymerization must possess a vinyl group bonded to one or two electron-withdrawing groups. For instance,...

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Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO₂ Beads for Protein Purification

Published on: November 19, 2018

Protein complexation with acrylic polyampholytes.

C S Patrickios¹, W R Hertler, T A Hatton

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Biotechnology and Bioengineering

|November 5, 1994

Summary

Protein-polyampholyte interactions were studied using turbidity measurements. Critical pHs near isoelectric points trigger precipitation, enabling efficient polymer recycling.

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Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

Biochemistry
Polymer Science
Materials Science

Background:

Proteins and synthetic polymers interact, influencing solution properties.
Understanding these interactions is key for applications like separation and recycling.
Polyampholytes offer unique properties due to their charged nature.

Purpose of the Study:

To investigate the interaction between proteins and ABC triblock methacrylic polyampholytes.
To determine the effect of pH on these interactions and subsequent precipitation.
To explore the potential for polymer recycling based on these interactions.

Main Methods:

Turbidimetric analysis of protein-polyampholyte mixtures at varying pH.
Measurement of protein precipitation yields.
Kinetic studies of precipitation using turbidity.
Analysis of polyampholyte self-aggregation.

Main Results:

Interactions occurred at critical pHs near the isoelectric points of proteins and polyampholytes.
Protein precipitation yields ranged from 10% to 90%.
Polyampholytes self-aggregate around their isoelectric point, affecting precipitation kinetics.

Conclusions:

Turbidity changes and precipitation yields indicate significant protein-polyampholyte interactions.
The pH-dependent behavior allows for controlled precipitation and potential polymer recovery.
This method offers an advantage for polymer recycling compared to homopolyelectrolytes.