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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Sporulation: an alternative way to recover recombinant proteins from Bacillus subtilis
G Maurizi1, P Bossù, D Boraschi
1Consorzio Biolaq, Via Campo di Pile, I-67100 L'Aquila, Italy.
Abstract:
A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-Ira) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.
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