Related Experiment Video
Updated: Jul 3, 2026
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
Published on: December 8, 2017
The Use of RP-HPLC for measuring activation and cleavage of rFVIIa during purification
I Mollerup1, S Bayne, E Halkjaer
1Biopharmaceuticals Division, Novo Nordisk A/S, Gentofte, Denmark.
Abstract:
A reverse phase HPLC (RP-HPLC) method for analysis of recombinant factor VIIa (rFVIIa) has been developed. The method discriminates between different forms of recombinant FVII (rFVII). To obtain separation of these closely related molecules the method has been optimized with respect to gradient profile and temperature. The method has been used for optimization of purification processes and for kinetic studies. EVidence for autolytic cleavage was obtained. (c) 1995 John Wiley & Sons, Inc.
