Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Passive Diffusion: Overview and Kinetics01:17

Passive Diffusion: Overview and Kinetics

Passive diffusion is a critical process that allows small lipophilic drugs to cross the cell membrane along a concentration gradient. This mechanism's efficiency depends on four primary factors: the membrane's surface area, the drug's lipid-water partition coefficient, the concentration gradient, and the membrane's thickness.
When administered orally, drugs establish a substantial concentration gradient between the gastrointestinal (GI) lumen and the bloodstream, expediting their diffusion into...
Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Drug Product Performance: In Vitro–In Vivo Correlation01:20

Drug Product Performance: In Vitro–In Vivo Correlation

In pharmaceutical development, it's crucial to establish a predictive in vitro–in vivo correlation (IVIVC) for two or more formulations to gain a comprehensive understanding of release properties. IVIVC reduces the need for costly in vivo studies and facilitates the establishment of meaningful dissolution specifications with significant cost savings and decreased regulatory burden. Furthermore, a meaningful IVIVC should predict Cmax and AUC within 20%, aligning with FDA guidance while adhering...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment and evaluation of a smoking cessation programme on the prevalence and intensity of smoking during a 6-month military deployment.

BMJ military health·2025
Same author

COVID-19: responding to a pandemic on Operation TORAL.

BMJ military health·2021
Same author

Control of three gastrointestinal illness outbreaks in a British Role 1 facility in Afghanistan: a primary care perspective.

BMJ military health·2020
Same author

UK Role 4 military infectious diseases and tropical medicine cases in 2005-2013.

Journal of the Royal Army Medical Corps·2017
Same author

Scaling of heat transfer and temperature distribution in granular flows in rotating drums.

Physical review. E·2016
Same author

Methods for the study of nerve cell migration and patterning.

Methods in molecular medicine·2011
Same journal

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species.

Biotechnology and bioengineering·2026
Same journal

Artificial Intelligence-Powered Algal Biodiesel: The Future of Biofuel Production Through Data-Driven Biotechnology.

Biotechnology and bioengineering·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2026

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

A simple correlation for predicting effective diffusivities in immobilized cell systems.

M R Riley1, F J Muzzio, H M Buettner

  • 1Department of Chemical and Biological Engineering, Rutgers University, P.O. Box 909, Piscataway, New Jersey 08855-0909.

Biotechnology and Bioengineering
|January 20, 1996
PubMed
Summary
This summary is machine-generated.

A new correlation method accurately predicts effective diffusivities in heterogeneous materials like immobilized cell systems. This method uses one measurement to estimate diffusion across all cell volume fractions, simplifying complex calculations.

More Related Videos

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Related Experiment Videos

Last Updated: Jul 3, 2026

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Area of Science:

  • Chemical Engineering
  • Biochemical Engineering
  • Materials Science

Background:

  • Predicting effective diffusivities in heterogeneous materials is crucial for process design.
  • Immobilized cell systems present complex diffusion challenges due to their varied structure.

Purpose of the Study:

  • To develop a simple correlation method for predicting effective diffusivities.
  • To validate the method using published data from immobilized cell systems.

Main Methods:

  • A single diffusivity measurement at a specific cell volume fraction is used.
  • The correlation is applied to predict diffusivities across a range of cell volume fractions.

Main Results:

  • The method accurately predicts effective diffusivities for small molecules in immobilized cell systems.
  • The correlation demonstrated high accuracy across 20 diverse datasets.
  • The method is applicable to any heterogeneous material with known phase properties.

Conclusions:

  • The developed correlation offers a simplified approach to diffusivity prediction.
  • This method can be broadly applied to various heterogeneous materials.
  • It reduces the need for extensive experimental measurements.