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Published on: June 24, 2025
Simultaneous single-tube PCR-based assay for the direct identification of the five most common meningococcal
Zacharoula Drakopoulou1, Konstantinos Kesanopoulos, Maria Sioumala
1National Meningitis Reference Laboratory, National School of Public Health, Athens, Greece.
Abstract:
This study presents a stepdown multiplex PCR assay for the simultaneous detection of the five most common Neisseria meningitidis serogroups (A, B, C, W-135 and Y) in 530 clinical samples obtained from 428 patients (271 blood and 259 cerebrospinal fluid). The sensitivity and the specificity was calculated to 100% [positive predictive value 100% (95%, CI 99.0-100%) and negative predictive value 100% (95% CI 99.0-100%)]. The overall effectiveness permits the rapid, accurate and inexpensive detection of the five most prevalent meningococcal serogroups in clinical samples. It is potentially a valuable tool for diagnosis and epidemiological monitoring of disease due to N. meningitidis.