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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

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Related Experiment Video

Updated: Jul 3, 2026

Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Bacteria detection by flow cytometry.

Oliver Karo1, Alexandra Wahl, Sven-Boris Nicol

  • 1Paul Ehrlich Institute, Langen, Germany.

Clinical Chemistry and Laboratory Medicine
|July 16, 2008
PubMed
Summary
This summary is machine-generated.

Rapid flow cytometry effectively detects bacteria in platelet concentrates, enhancing transfusion safety. This method improves sensitivity and practicality for bedside bacterial screening, preventing transfusion-related sepsis.

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Imaging Flow Cytometry to Study Microbial Autoaggregation
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Imaging Flow Cytometry to Study Microbial Autoaggregation

Published on: September 29, 2023

Area of Science:

  • Transfusion Medicine
  • Microbiology
  • Analytical Chemistry

Background:

  • Bacterial contamination in blood components, especially platelet concentrates, poses a significant risk in transfusion medicine.
  • Platelet concentrates' storage conditions facilitate bacterial multiplication, increasing infection risk.
  • Current bacterial detection methods have limitations, leading to potential transfusion-transmitted sepsis.

Purpose of the Study:

  • To develop and optimize flow cytometry protocols for routine bacterial detection in platelet concentrates.
  • To evaluate the impact of improved reagents and simplified procedures on method sensitivity and practicability.
  • To demonstrate the implementation of fluorescent absolute count beads for accurate bacterial quantification.

Main Methods:

  • Flow cytometry was adapted for rapid bacterial detection in platelet concentrates.
  • Improved test reagents and a simplified pre-incubation procedure were evaluated to enhance sensitivity.
  • Fluorescent absolute count beads were used as an internal standard for accurate quantification.
  • A modified culture method using flow cytometry was explored.

Main Results:

  • Flow cytometry demonstrated feasibility and rapidity for detecting bacteria in platelet concentrates.
  • Optimized protocols and reagents improved the method's practicability and sensitivity.
  • The use of internal standards ensured reliable bacterial quantification.
  • The adapted culture method showed potential for enhanced detection.

Conclusions:

  • Flow cytometry offers a rapid and sensitive approach for bacterial detection in platelet concentrates.
  • Optimized protocols enhance the method's suitability for routine clinical application.
  • This technology can significantly improve transfusion safety by preventing bacterial contamination-related adverse events.