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Published on: April 22, 2022
Update on tools for evaluation of uridine diphosphoglucuronosyltransferase polymorphisms
Upendra A Argikar1, Otito F Iwuchukwu, Swati Nagar
1Novartis Institutes for BioMedical Research, Inc., 250 Massachusetts Avenue, Cambridge, MA 02139, USA.
Evaluating uridine diphosphoglucuronosyltransferase (UGT) genetic variations requires diverse tools. This review covers methods for assessing UGT genotype-phenotype correlations, crucial for drug metabolism studies.
Area of Science:
- Pharmacology and Drug Metabolism
- Genetics and Genomics
Background:
- The UGT enzyme superfamily is vital for metabolizing endogenous and exogenous compounds.
- Genetic variations (polymorphisms) are common across UGT family members, impacting drug efficacy and safety.
Purpose of the Study:
- To review and discuss the available tools for evaluating UGT polymorphisms.
- To highlight methods for assessing the relationship between UGT genotype and phenotype.
Main Methods:
- A comprehensive literature search was conducted.
- Included studies focused on in vitro methods for UGT polymorphism evaluation.
Main Results:
- Various methods for UGT genotype-phenotype correlation studies are presented.
- Techniques include using transfected mammalian cells, genotyped human liver tissue, RNA inhibition, and transgenic animal models.
Conclusions:
- Established and novel techniques facilitate the preclinical evaluation of UGT genotype-phenotype relationships.
- Challenges and future opportunities in this field are discussed.
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