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Updated: Jul 31, 2026
08:48
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
[In situ hybridization in human genome mapping].
Tsitologiia I Genetika
|March 1, 1991
Summary
Non-radioactive in situ hybridization offers a safer, sensitive method for gene and chromosome localization. This technique is promising for analyzing DNA polymorphism in the human genome.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Context:
- In situ hybridization is a powerful technique for visualizing nucleic acid sequences within cells or tissues.
- Traditional methods often rely on radioactive probes, posing safety and disposal challenges.
- Advancements in non-radioactive labeling offer a viable alternative with significant advantages.
Purpose:
- To discuss the methodological advancements and applications of non-radioactive in situ hybridization.
- To highlight the benefits of non-isotope probe labeling over radioactive methods.
- To explore strategies for enhancing the sensitivity of non-radioactive in situ hybridization.
Summary:
- This review covers the principles and practical aspects of non-radioactive in situ hybridization (ISH).
- It details various labeling compounds and methods for improving sensitivity.
- The application of non-radioactive ISH for gene and chromosome localization is discussed, with reported results.
- The potential for molecular-genetic analysis of human DNA polymorphism using this technique is emphasized.
Impact:
- Provides a comprehensive overview of non-radioactive ISH, facilitating its adoption in research.
- Offers a safer and potentially more sensitive alternative to radioactive ISH.
- Supports advancements in human genome research, particularly in DNA polymorphism analysis.
Related Concept Videos
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...